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Tandem Recombinant Plasmid Construction as Positive Control for PIK3CA H1047R Detection Based on SYBR Green I qPCR

Tandem Recombinant Plasmid Construction as Positive Control for PIK3CA H1047R Detection Based on SYBR Green I qPCR

Nahdaturrugaisiyah, Azamris, Bugi Ratno Budiarto, I Made Artika, Desriani (2018)

Pak. J. Biotechnol., Vol. 15 (3): 735-742.

 Tandem Recombinant Plasmid Construction as Positive ControlPIK3CA H1047R mutation is found in breast cancer in high frequency and its detection could be applied as prognosis and predictive factor for trastuzumab therapy. qPCR is one of the simplest and robust method for PIK3CA H1047R detection. Provision of positive control for PIK3CA H1047R detection based on qPCR will support data analysis efficiently and avoid false negative result. In this research, we constructed a tandem recombinant plasmid (pGEM-tandPIK3CA) as positive control for Tm Shift SYBR Green I qPCR-based of PIK3CA H1047R detection system by ligating wild-type and PIK3CA H1047R fragments tandemly into pGEM-T Easy. The tandem plasmid was confirmed by restriction, DNA sequencing and qPCR. As a result, pGEMtandPIK3CA has been successfully constructed and confirmed. Statistical analysis shows high repeatability and reproducibility with % CV of <25%. The main advantage of this tandem positive control is its ability to serve as positive control for both wild-type PIK3CA and PIK3CA H1047R simultaneously, therefore improving the efficiency of the detection system.

Keywords: Breast cancer, PIK3CA H1047R, qPCR, positive control.

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3475&bid=16075

Disusun oleh: Ludya AB/Pustakawan

 

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Antimicrobial Activity of Selenium Nanoparticles Synthesized by Actinomycetes Isolated from Lombok Island Soil Samples

Antimicrobial Activity of Selenium Nanoparticles Synthesized by Actinomycetes Isolated from Lombok Island Soil Samples

Shanti Ratnakomala, Nurul Fitri Sari, Fahrurrozi and Puspita Lisdiyanti (2018)

Jurnal Kimia Terapan Indonesia. Vol. 20 (1): 8-15. e-ISSN: 2527–7669.

 Antimicrobial Activity of Selenium NanoparticlesA total of 98 actinomycetes were isolated from the soil and litter samples collected from the cacao and coffee plantation in Lombok Island, West Nusa Tenggara of Indonesia. These isolates were screened for their antimicrobial activity. Among 98 isolated strains, only 24 isolates showed antimicrobial activity against test microorganisms, 20.4% were active against Bacillus subtilis BTCC B-612, 14.3% against Staphylococcus aureus BTCC B-611, and 5.1% against Escherichia coli BTCC B-609. Of these 24 isolates, 3 were found to be able to grow in medium containing 3 mM Selenium oxide and the culture were changed color to red. Two of the best strains, L-155 and L-156, were selected for assessing production of Selenium nanoparticles. Bioreduction of selenium nanoparticles was confirmed by UVvisible spectrophotometer that showed the peak between 300 and 320 nm. Biosynthesized selenium nanoparticle from isolate actinomycetes L-155 and L-156 were found to have a broad spectrum of activity against the tested microorganisms Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Micrococcus luteus, and Candida albicans. The study shows the rapid and eco-friendly synthesis of selenium nanoparticles from soil actinomycetes. Most of these active isolates showed to possessed antibacterial property.

Keywords: Lombok Island, actinomycetes, selenium nanoparticles, antimicrobial activity

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3472&bid=16045

Disusun oleh: Ludya AB/Pustakawan

 

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Kebun Plasma Nutfah Puslit Bioteknologi LIPI dikunjungi SD Plus Cerdas Alam Rizkia

Cibinong, Humas LIPI. Pusat Penelitian Bioteknologi Lembaga Ilmu Pengetahuan Indonesia (LIPI), pada (Kamis 10/10) menerima kunjungan SD Plus Cerdas Alam Rizkia Bogor, Rombongan yang berjumlah 54 orang terdiri dari 45 orang siswa dan 9 orang guru pendamping. Rombongan berkunjung ke kebun plasma nutfah dan mendapatkan informasi tentang kegiatan yang dilakukan  dirumah kasa model hidroponik sayuran dan model budidaya sayuran organik. “Sayuran organik dibudidayakan secara alami tanpa tambahan bahan kimia. Media tanam yang menggunakan pupuk, pemillihan bibit, proses tanam, pemeliharaan dan pembasmian hama tidak menggunakan bahan kimia yang notabene dapat mengganggu kesehatan” ungkap, Heru Wibowo yang merupakan salah seorang  teknisi di Laboratorium Agronomi untuk Evaluasi Produk Bioteknologi Pusat Penelitian Bioteknologi LIPI.

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