Kecepatan Regenerasi Kalus Somatik Embriogenik Terung Pada Beberapa Media Maturasi

Hartati, Hanifah Agani, N. Sri Hartati, Enny Sudarmonowati (2018)

Jurnal ILMU DASAR, Vol.19 (2): 125-134. ISSN-2442-5613

Ralstonia solanacearum is one of the most important pathogen that causes bacterial wilt disease in eggplant and inhibits eggplant production. Improvement of eggplant varieties resistant to bacterial wilt can be accomplished through genetic manipulation. Regeneration of in vitro plants isone of the important tools to supports plant improvementthrough biotechnology. This study was aimed to determine the rate of eggplant regeneration in various maturation media, and to find the best medium for eggplant regeneration based on maturation rate and the number of cotyledon produced. We used resistant eggplant (accession 032)as the material to produce somatic embryogenic.There were 7 types of regeneration media used in this research. MS medium was supplemented with a certain concentration of plant growt regulators , such as: 1 mg / L + BAP 1 mg / L, NAA 4mg / L, TDZ 0.005 mg / L, TDZ 0.001 mg / L, CuSO4 2mM + BAP 1 mg / L, CuSO4 2mM + BAP 2 mg / L and Kinetin 1 mg / L + CuSO4 2mM. Three clumps of callus per plate with three replications were transferred to MS suplemented medium. The parameters observed were the color of callus before and after they were transfered to regeneration medium, the day of formation of globular, heart-shaped, tubular and cotyledonary phase, and the number of cotyledons formed. The results obtained showed the somatic embryogenic color of the 032 genotype was white with friable structure before being transferred to regeneration medium and was turned to yellowish white after being transferred to the regeneration medium. On the day sixth, friable embryogenic somatic of eggplant was developed into nodule on medium MS + NAA 4 mg / L, MS + CuSO4 2mM + BAP medium 1 mg / L, and MS + CuSO4 2mM + BAP 2 mg / L. Somatic embryogenic callus of accession 032 were able to pass complete globular, heart-shaped, tubular and cotyledonary phase. The most responsive medium for somatic embryogenic callus regeneration, based on the days of the callus phases formation and the number of early-phase cotyledons obtained, were MS medium suplemented with CuSO4 2mM + BAP,and CuSO4 2 mM + BAP 2 mg / L.

Keywords: eggplant, Ralstonia solanacearum, regeneration, cotyledonary, clump, BAP

Katalog: http://perpus.biotek.lipi.go.id/perpus/index.php?p=fstream&fid=3486&bid=16092

Disusun oleh: Ludya AB/Pustakawan

Low cost and comprehensive pork detection in processed food products with a different food matrix

Fenny Aulia Sugiana, Henni Widyowati, M. Ali Warisman, Suryani, and Desriani (2018)

Indonesian Journal of Biotechnology, 23 (1): 21-27.ISSN 0853-8654

The adulteration of processed beef-based meat products with pork is a sensitive issue in Indonesia. Therefore a simple, low cost, and accurate method is required for the detection of pork, so as to protect consumers from accidental consumption of adulterated meat. In this study, we developed a detection method for the low cost identification of pork in processed meat products. We used the cost-efficient Taq DNA polymerase, DreamTaq Green PCR master mix (2x), and duplex PCR method to recognize pork simultaneously with 18S rRNA detection. A positive control containing a pork gene inserted into pGEM®-T easy was prepared, along with a negative control. The results of the duplex PCR were used to assess its specificity, detection limit, and its ability to recognize pork in processed meat products with a different food matrix. 18S rRNA detection was for confirming DNA integrity of DNA extracted from the processed food, while the positive control confirmed that the reagents were working well and the negative control confirmed a non-contamination problem. Following this, the duplex PCR was opধmized and the optimum concentration primer for duplex PCR detection was found to be 3 µM for pork and 0.2 µM for 18S rRNA. As liħle as 3.125 ng of the DNA template could be used to detect whether a sample contained pork. Of the nine commercial processed meat products tested, five were found to contain pork while four halal products showed no signs of pork. It can be concluded that duplex PCR is a simple, fast, sensitive, specific, and low cost method of detecting pork in processed meat products.

Keywords: 18S rRNA, duplex PCR, low cost, pork, processed meat

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3480&bid=16089

Disusun oleh: Ludya AB/Pustakawan