• apec2019a
  • dputiapec2019a
  • biotekastra-3 1
  • kerjasamabalitri
  • Annual Meeting and Seminar 2019 : APEC Reserach Center for Advanced Biohydrogen Technology,July 26, 2019 at Ekakarya Botanical Garden, Bedugul, Bali
  • Deputy of Life Sciences at Annual Meeting and Seminar 2019 : APEC Reserach Center for Advanced Biohydrogen Technology,July 26, 2019 at Ekakarya Botanical Garden, Bedugul, Bali
  • Perkuat Kemitraan, LIPI Terima Kunjungan Sains PT Astra International, TBK.
  • Pusat Penelitian Bioteknologi Lipi Jalin Kerjasama Dengan Balai Penelitian Tanaman Industri Dan Penyegar Kementrian Pertanian

bioteknologi update a

seputar biotek web

biotek media

Print

Isolation and characterization of mannanase, xylanase, and cellulase from marine bacteria Bacillus sp.

Yopi, Apridah Cameliawati Djohan, Nanik Rahmani, Alifah Mafatikhul Jannah (2017).

Biofarmasi (Rumphius J NatProd Biochem), 15 (1): 15-20

Microbial endophite from plant sugar-apple 1Isolation, identification, and characterization of mannanase, xylanase and cellulase producing indigenous marine bacteria have been conducted from total 20 isolates. Based on 16S rDNA sequence analysis, three potential isolates are obtained and identified as Bacillus subtilis (M8), Bacillus tequilensis (X4) and Bacillus cereus (C9). The potential strains M8, X4 and C9 can produce mannanase, xylanase and cellulase activities such as 9.5 U/mL; 0.36U/mL;0.56U/mL with optimum pH and temperature 6.0;50°C, 5.5;70°C and 8;50°C, respectively. Based on the TLC analysis, mannanase from M8 and xylanase from X4 has potential to hydrolyzed mannan and xylan for producing oligosaccharides with size around tri-hexasaccharides as a main product.

Kata kunci: Bacillus sp.,cellulase, hemicellulase, marine bacteria, oligosaccharide

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3309&bid=15784

Disusun oleh: Ludya AB/Pustakawan

Print

Sequential Adaptation in Mammalian CHO-K1 Cells Producing Human Erythropoietin

 Popi Hadi Wisnuwardhani, Endah Puji Septi Setyani

 Annales Bogorienses, Vol.21 , No.1 (2017) : 15-20

Sequential_Adaptation_in_Mammalian_CHO-K1_Cells_Producing_Human_ErythropoietinThe production of recombinant proteins for clinical applications using mammalian cell technology has become a prevalent system because of its capacity in assembling functional proteins. One of the main problems with CHO-K1 cells is that this cell has to grow in the presence of serum. However, the presence of serum will complicate the downstream step for protein production. Thus, protein produced in media without serum, theoretically, would be easier to purify. Technically, this type of cell can be produced by growing the CHO-K1 cells in serum-free media by using adaptation method in suspension condition. This research showed that through sequential adaptation using conditioned media, the CHO-K1 cell line that produces the human erythropoietin gene (hEPO) was able to grow in suspension culture using serum-free media. Based on Western blot analysis, it showed that the protein (hEPO) was able to be expressed in suspension culture with molecular mass of about 47 kDa.

Video Bioteknologi LIPI


Lock full review www.8betting.co.uk 888 Bookmaker

Seputar Biotek

Pusat Penelitian Bioteknologi-LIPI menerima Kunjungan Universitas Halu Oleo, Kendari

HOUCibinong, 13 Agustus 2019.Keingintahuan masyarakat akan penelitian dibidang bioteknologi akhir-akhir ini semakin meningkat, hal ini diketahui berdasarkan permintaan kunjungan ilmiah ke Pusat Penelitian Bioteknologi LIPI. Bioteknologi merupakan salah satu bidang ilmu Pengetahuan yang sangat menarik untuk dipelajari karena, berbagai solusi pada permasalahan yang saat ini timbul dapat terselesaikan dengan bioteknologi seperti melalui teknologi rekayasa genetika, kultur jaringan, DNA rekombinan, pengembang biakan sel induk, kloning bahkan permasalahan tumpahan minyak dilaut. Pusat Penelitian Bioteknologi LIPI menerima kunjungan dari Universitas Halu Oleo Kendari, Jurusan Bioteknologi,sebanyak 32 orang. Rombongan diterima digedung auditorium dan diberikan informasi tentang P2 Bioteknologi sebelum berkunjung ke laboratorium.

Read more...

Laporan

 laptah2018 

cover rb2017 

lkj2018

kunjungan

pelatihan

pembimbingan

pengujian

Pusat Penelitian Bioteknologi LIPI