• esa unggul
  • kunjungan
  • kapusbiotek
  • apec2019a
  • dputiapec2019a
  • biotekastra-3 1
  • kerjasamabalitri
  • Kunjungan Universitas Esa Unggul Jurusan Bioteknologi ke Pusat Penelitian Bioteknologi-LIPI, Cibinong- 3 Desember 2019
  • Kunjungan SD Plus Cerdas Alam Rizkia ke Kebun Plasma Nutfah Pusat Penelitian Bioteknologi LIPI, Cibinong,10 oktober 2019
  • Pelantikan Dr. Puspita Lisdiyanti M. Agr. Chem sebagai Kepala Pusat Penelitian Bioteknologi LIPI dan Dr. Ahmad Fathoni sebagai Plt. Kabid Pengelolaan Penelitian Pusat Penelitian Bioteknologi LIPI Periode 2019-2024 oleh kepala Lembaga Ilmu Pengetahuan Indonesia di Jakarta, 10 September 2019.
  • Annual Meeting and Seminar 2019 : APEC Reserach Center for Advanced Biohydrogen Technology,July 26, 2019 at Ekakarya Botanical Garden, Bedugul, Bali
  • Deputy of Life Sciences at Annual Meeting and Seminar 2019 : APEC Reserach Center for Advanced Biohydrogen Technology,July 26, 2019 at Ekakarya Botanical Garden, Bedugul, Bali
  • Perkuat Kemitraan, LIPI Terima Kunjungan Sains PT Astra International, TBK.
  • Pusat Penelitian Bioteknologi Lipi Jalin Kerjasama Dengan Balai Penelitian Tanaman Industri Dan Penyegar Kementrian Pertanian

bioteknologi update a

seputar biotek web

biotek media

Print

Improvement of HER2I655V TARMS-PCR Performance by DNA Quality Analysis

Improvement of HER2I655V TARMS-PCR Performance by DNA Quality Analysis

Bugi Ratno Budiarto, Azamris, and Desriani

Annales Bogorienses Vol. 21 (2): 52-62.

 tarmReliable TARMS-PCR is a prerequisite in constructing a solid conclusion in genetic diagnostics. The validity of data generated by this molecular technique in most cases is hampered by a false positive result. In attempt to develop a TARMS-PCR for HER2I655V genotyping with no interfering of bias we used DNase I to eliminate DNA contaminant resided in PCR reagent. TARMS-PCR with no enzymatic pretreatment on PCR master mix kit produced a false positive result on HER2I655V TARMS-PCR using as recombinant plasmids system model proven by the presence of multiple PCR products in Non-Template Control (NTC). A dose of 0.1 U of the enzyme could eliminate this DNA contaminant effectively, although this pretreatment altered the specificity of HER2I655V TARMS-PCR genotyping on certain genotype. Combination of touchdown TARMS-PCR with another allelespecific primer recovered specificity of detection on this model system. Interestingly, this optimized HER2I655V TARMS-PCR can only be used for genotyping the clinical samples if only further optimization was done using genomic DNA as template.

Keywords: TARMS-PCR, HER2I655V, DNase I, Polymorphism

Katalog: http://http://jurnal.biotek.lipi.go.id/index.php/annales/article/view/308/pdf

Disusun oleh: Ludya AB/Pustakawan

 

Print

Optimization of Substrate and Starter Cell Concentrations for Dibenzothiopene Biodegradation by Indigeneous Marine Bacteria Mauricauda olearia LBF-1- 0009, Alcanivorax xenomutants LBF-1-0018, and Stakelama pacifica LBF-1- 0031

Optimization of Substrate and Starter Cell Concentrations for Dibenzothiopene Biodegradation by Indigeneous Marine Bacteria Mauricauda olearia LBF-1- 0009, Alcanivorax xenomutants LBF-1-0018, and Stakelama pacifica LBF-1- 0031

Elvi Yetti, Ahmad Thontowi, and Yopi

Annales Bogorienses Vol. 21, No. 2: 38-44

cellDibenzothiophene (DBT) and its derivatives are widely used-model of organic sulfur compounds for the biodegradation process of petroleum oil. The abilities of microorganisms to degrade pollutants are significantly influenced by various factors such as microbial species, nutrients and environmental parameters. In this research, we conducted a follow-up study to determine the optimum conditions of two parameters affecting DBT biodegradation, namely substrate and starter cell concentrations. Three indigenous marine bacteria isolated from Indonesia’s sea environments potentially degrading DBT were examined. The isolates are belong to Mauricauda olearia strain CL-SS4 (99%), Alcanivorax xenomutants strain JC109 (99%), and Stakelama pacifica strain JLT832 (99%). The optimum DBT concentrations act as the growth substrate for all three isolates was 100 ppm, while the optimum cell concentrations for starter inocula was 20 of OD600 nm conversion units.

Keywords: optimization, substrate concentration, starter cell concentration, dibenzothiophene biodegradation, marine bacteria

Katalog: http://jurnal.biotek.lipi.go.id/index.php/annales/article/view/300/pdf

Disusun oleh: Ludya AB/Pustakawan

 

 

Video Bioteknologi LIPI


Techno Park Banyumulek 2017 

Lock full review www.8betting.co.uk 888 Bookmaker

Seputar Biotek

MAHASISWA UNIVERSITAS AHMAD DAHLAN YOGYAKARTA KUNJUNGI PUSAT PENELITIAN BIOTEKNOLOGI LIPI

 

uad1Cibinong, 28 November 2019Keingintahuan masyarakat akan penelitian dibidang bioteknologi akhir-akhir ini semakin meningkat, hal ini dapat dilihat dengan banyaknya permintaan kunjungan ilmiah ke Pusat Penelitian Bioteknologi LIPI. Pusat Penelitian Bioteknologi LIPI menerima kunjungan dari Universitas Ahmad Dahlan Yogyakarta sebanyak 91 orang. Kegiatan kunjungan ini diterima di Gedung Bioteknologi Peternakan dan dibuka dengan pengenalan dan pemutaran video Profil Pusat Penelitian Bioteknologi-LIPI dan dilanjutkan tur ke laboraorium.

Tujuan dari kunjungan ini adalah selain sebagai kegiatan rutin diluar kampus juga untuk memperdalam ilmu pengetahuan mahasiswa terkait bidang Bioteknologi dengan melihat secara langsung laboratorium yang ada di Pusat Penelitian Bioteknologi-LIPI. Pada kunjungan kali ini para peserta mengunjungi Laboratorium Genetika Molekuler dan Modifikasi Jalur Biosintesis Tanaman, Laboratorium Biokatalis dan Fermentasi dan Laboratorium Biak Sel dan Jaringan Tanaman.

Read more...

Laporan

 laptah2018 

cover rb2017 

lkj2018

kunjungan

pelatihan

pembimbingan

pengujian

Pusat Penelitian Bioteknologi LIPI