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Stabilization of luciferase from Renilla reniformis using random mutations

Megumi Shigehisa, Norie Amaba, Shigeki Arai, Chisato Higashi, Ryo Kawanabe, Ayano Matsunaga, Fina Amreta Laksmi, Masao Tokunaga and Matsujiro Ishibashi

renillaWe expressed luciferase (RLuc) from Renilla reniformis in Escherichia coli. RLuc was purified using a Ni-NTA column and subsequently characterized. It was unstable in acidic solutions and at 30°C. To increase the stability of RLuc, the Rluc gene was randomly mutated using error-prone polymerase chain reaction. E. coli harboring the mutated gene was screened by detecting luminescence on a plate containing the substrate coelenterazine at 34°C. Three mutants, i.e. N264SS287P, N178D and F116LI137V, were obtained. The solubilities and specific activities of these mutants were higher than those of the wild type. Furthermore, the N264SS287P mutant maintained stability at a temperature approximately 5°C higher than that of the wild type, while denaturation of the F116LI137V mutant started at a temperature that was 5°C lower than the wild type, and ended at a temperature that was 7°C higher. We examined the obtained mutations using thermal shift assays and a computer program Coot in this study.

Keywords: Mutation, Stabilization, error-prone PCR, luciferase, Renilla reniformis

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3143&bid=15591

Disusun oleh: Ludya AB/Pustakawan

 

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Aktivitas Antibakteri Aktinomisetes Laut Dari Pulau Enggano [Antibacterial activity of marine actinomycetes from Enggano Island]

Shanti Ratnakomala, Pamella Apriliana, Fahrurrozi Fahrurrozi, Puspita Lisdiyanti, Wien Kusharyoto

Berita Biologi, Volume 15 Nomor 3, Desember 2016, 275-283. ISSN 0126-1754

ntibacterial activity of marine actinomycetes from Enggano IslandMarine actinomycetes were isolated from mangrove sediment from the coast in Enggano, Bengkulu Province, Indonesia using the medium NBRC No. 802 modified by the addition of 2% NaCl. A total of 29 isolates of actinomycetes were isolated from three mangrove sediment samples and evaluated their potential to produce bioactive metabolites. Screening of 29 isolates marine actinomycetes isolates were performed against three bacterial pathogens had been done. Bacteria test used was Escherichia coli NBRC 14 237, Staphylococcus aureus NBRC13 276, and Bacillus subtilis NBRC 3134. Screening result showed that seven isolates have inhibitory effects against bacteria test and 22 other isolates have no inhibition. Of the seven isolates, one isolate has inhibitory effect against the growth of Gram-negative bacteria Escherichia coli, while six other isolates inhibit Gram-positive bacteria Bacillus subtilis and Staphilococcus aureus. It was concluded that, of the 29 isolates conducted in the experiment, seven isolates produce antibacterial compounds on agar medium. Molecular identification of 23 isolates were identified based on the gene 16S RNA sequences showed that 22 isolates belong to the genus Streptomyces and one strain belongs to the genus Dermacoccus.

Keywords: marine actinomycetes, antibacterial, Enggano Island

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3371&bid=15841

Disusun oleh: Ludya AB/Pustakawan

 

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Production of Manooligomannan from Palm Kernel Cake by Mannanase Produced from Streptomyces Cyaenus

Awan Purnawan, Yopi, Tun Tedja Irawadi (2017)

Biosaintifika: Journal of Biology & Biology Education, 9(1), 73-80. p-ISSN 2085-191X

BiosaintifikaThe increase of public attention to health has prompted researchers to look for new sources of functional food. Palm Cake Kernel (PKC) waste was abundant in Indonesia, Oligosaccharide has an important benefit for human health. Recently oligosaccharide is not only important as an artificial sweetener, but also as a functional food component. This study was aimed to produce oligo-mannan enzymatically from PKC waste using mannanase derived from of Streptomyces cyaenus isolates of indigenous Indonesia. The enzyme concentration was determined by enzyme activity assay while oligo-mannan content in the PKC was analyzed using TLC and HPLC. Mannanase enzyme activity of 1706 U/ml on the second day of agitation 200 rpm at a temperature of 30°C Hydrolysis of mannooligomannan by using mannanase produced by streptomyces cyaenus. The optimum mannanase enzyme activity obtained on day 2 with the value of the activity as much of 0.702 U/mL. The protein content of the 2nd day at an agitation speed of 150 rpm, 200 rpm, and 250 rpm, respectively, were 1783, 1950 and 2283 ppm. Streptomyces cyaenus is Indonesian original isolates potentially producing mannanase that can produce mannooligomannan

Keywords: Palm Kernel Cake (PKC); Streptomyces cyaenus; mannanase enzyme; mannooligosaccharide

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3370&bid=15840

Disusun oleh: Ludya AB/Pustakawan

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Body Weight Estimation of Bali Cattle in Banyumulek Techno Park, West Nusa Tenggara using Several Morphometric Parameters

Paskah Partogi Agung, Widya Pintaka Bayu Putra, Saiful Anwar, and Ari Sulistyo Wulandari.

Buletin Peternakan 42 (1): 20-25, February 2018

Mean valueThis study was conducted to find the best linear regression equation to estimate the body weight (BW) of female Bali cattle in Banyumulek Techno Park, West Nusa Tenggara based on three morphometric parameters, i.e. withers height (WH), body length (BL), and heart girth (HG). Data collection was conducted by random sampling of female Bali cattle (1-5 years of age) in Banyumulek Techno Park. A total of 63 female Bali cattle were used in this study. The parameter of WH (distance from the ground level to the highest point of withers) and BL (distance from the shoulder joint (later tuberosity of humerus) to the pelvic bone) were measured with a measuring stick. The HG data (circumference around the chest at the fourth rib) was measured with a measuring tape. Identification of age was carried out based on the animal recording book and incisors condition. The descriptive statistic analysis (mean value, standard deviation, the coefficient of variation, minimum and maximum value) of the morphometric and BW data were performed using MS Excel 2017 program. The linear regression equation analysis and the comparison between actual and estimated BW values (Independent sample T-test) were performed using SPSS 16.0 program. The BL and BW parameters had the highest correlation coefficient (r) value (0.86). The test result showed that the predicted BW value of female Bali cattle was not significantly different with the actual BW when using G model of linear regression equation (three variables used). The G model of linear regression equation had the highest R2 value (0.84). However the R2 value in the F model with two variables (BL and HG) was also high (0.83). It can be concluded that the simple linear regression equation BW=(0.96)(WH)+(1.26)(BL)+(1.57)(HG)-284.92 are the best model to estimate the BW value of female Bali cattle in Banyumulek Techno Park.

Keywords: Bali cattle, Body measurement, Body weight, Correlation, Regression

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3340&bid=15815

Disusun oleh: Ludya AB/Pustakawan

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Antioxidant Production Of Lactic Acid Bacteria Isolated From Indonesian Traditional Fermented Buffalo Milk (Dadih)

Hasim, AZ Mustopa, N Andrianto, Fatimah, Didah Nur Faridah (2017).

Journal of Pharmacy and Biological Sciences, Vol. 12 (5): 76-82. ISSN 2278-3008.

Microbial endophite from plant sugar-apple 1One of antioxidant sources is foods that contain peptides from enzymatic hydrolysis and/or fermentation by lactic acid bacteria (LAB). The availability of antioxidants in fermented food, particularly milk, is associated with the presence of LAB. Dadih is traditionally made by keeping the fresh raw buffalo milk in a bamboo tube capped with banana leaf and allowing it to ferment spontaneously at room temperature for two days. This research aimed to obtain bacteria from dadih with the highest antioxidants. Isolate Lac 3 and Lac 13 displayed the highest antioxidant activity, with inhibition capacity of 64.2 persen and 63.79 persen respectively against DPPH radical. The result showed that 6 best isolates (Lac 3, Lac 13, DS 6, DS 10, DS 12, and DS 14) were not significantly different. Lac 3 was found to be identical with Lactococcus lactic subsp lactic, while Lac 13 was identical with Lactobacillus plantarum with the percentage of similarity of 98 persen and 96 persen respectively. Lactococcus lactic subsp lactic displayed the highest antioxidant activity at the early stationary phase and at the lowest pH value of culture.

Kata kunci: antioxidant activity, dadih, lactic acid bacteria, milk

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3338&bid=15813

Disusun oleh: Ludya AB/Pustakawan

 

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