• banyumulek4
  • banyumulek5
  • banyumulek
  • kakao
  • fgd
  • fgd
  • fgd
  • science sma
  • isibio
  • kunjungan
  • biotek-phapros
  • Kunjungan dan diskusi kerja sama Techno Park Banyumulek NTB di lokasi PT Gerbang NTB Emas, mitra Pusat Penelitian Bioteknologi-LIPI. Mataram, 12 Oktober 2017
  • Kegiatan Praktik Kerja Lapangan Pemanfaatan Tanaman Lokal guna Bahan Tambahan Pembuatan Pupuk Organik Cair di Lokasi Agro Edu Wisata Techno Park Banyumulek, Lombok, Nusa Tenggara Barat, 10 Oktober 2017
  • Kegiatan kunjungan dan konsultasi Praktik Kerja Lapangan para Mahasiswa S1 Fakultas Peternakan Universitas Mataram ke Kawasan Agro Edu Wisata Techno Park Banyumulek Nusa Tenggara Barat, Lombok Barat, 9 Oktober 2017
  • Kegiatan dengar pendapat antara Kelompok Tani Kopi dan Kakao Desa Genggelang KLU dengan Komisi XI DPR RI, Direktorat Jenderal Perkebunan (Dirjen. Bun.) Kementerian Pertanian Republik Indonesia, Balai Besar Perbenihan dan Proteksi Tanaman Perkebunan (BBPPTP) Medan, Dinas Pertanian dan Perkebunan Provinsi Nusa Tenggara Barat (NTB), Dinas Ketahanan Pangan dan Pertanian KLU, Fakultas Pertanian Universitas Mataram dan Peneliti Pusat Penelitian Bioteknologi-LIPI. Kegiatan ini dilaksanakan di Aula Kelompok Petani Kakao ‘Bunga Mekar’, Dusun Senara, Desa Genggelang, Kecamatan Gangga, KLU, pada 13 September 2017. Pada kegiatan ini, Peneliti Mikrobiologi Terapan Pusat Penelitian Bioteknologi-LIPI, Dr. Fahrurrozi, memaparkan kegiatan kerja sama aplikasi IPTEK fermentasi biji kakao antara Pusat Penelitian Bioteknologi-LIPI dengan Dinas Ketahanan Pangan dan Pertanian, Kabupaten Lombok Utara, NTB.
  • Foto para pembicara pada acara Knowledge Sharing and Discussion dengan Judul "Status Terkini Penelitian dan Pengembangan Bioteknologi Pertanian", di Auditorium Puslit Bioteknologi LIPI-Cibinong, 8 September 2017,sebagai bagian dari rangkaian kegiatan Open House Puslit Bioteknologi LIPI 6-8 September 2017.
  • Foto para pembicara pada Focus Group Discussion (FGD) dengan Judul "Workshop Peran Riset dan Kebijakan Untuk Penguatan Rantai Nilai Ekonomi Ubi Kayu Indonesia", di Auditorium Puslit Bioteknologi LIPI-Cibinong, 7 September 2017,sebagai bagian dari rangkaian kegiatan Open House Puslit Bioteknologi LIPI 6-8 September 2017.
  • Foto bersama Kapuslit Bioteknologi LIPI, Dr. Ir. Bambang Sunarko bersama peserta FGD dengan Judul "Sinergi Konsorsium Kemandirian Bahan Baku Obat : Pengembangan Obat Malaria Dihidroartemisinin", di Auditorium Puslit Bioteknologi LIPI-Cibinong, 6 September 2017,sebagai bagian dari rangkaian kegiatan Open House Puslit Bioteknologi LIPI 6-8 September 2017.
  • Kegiatan Science in Touch, Praktik Isolasi dan Ekstraksi DNA bagi Siswa SMA pada Science Week Fair Kedeputian Bidang IPH LIPI, Cibinong, 4 September 2017. Kegiatan ini bertujuan untuk memperkenalkan bioteknologi bagi para siswa SMA sejak dini dengan cara yang menarik (fun and fearless). Terlihat peneliti bidang biologi molekuler tanaman, Dr. Wahyuni menerangkan apa itu DNA kepada para siswa dengan cara yang populer.
  • Penandatangan Nasakah Nota Kesepahaman (MoU) antara Pusat penelitian Bioteknologi-LIPI, Center for Innovation, Indonesian Institute of Sciences (PUSINOV LIPI), Kobe University, Bio-Energy Corporation, PT. Agricinal tentang Cooperation on Development and Implementation of integrated Biorefinery Technology in Indonesia, pada kegiatan International Symposium Innovative Bioproduction Indonesia 2017 (ISIBio 2017), disaksikan Kepala Badan Standardisasi Nasional (BSN) Prof. Dr. Bambang Prasetya dan Deputi Bidang Ilmu Pengetahuan Hayati LIPI, Prof. Dr. Enny Sudarmonowati, Bogor 27 September 2017
  • Kunjungan Direktur Perencanaan & Pengembangan PT Bio Farma (Persero), drh. Sugeng Raharjo, MM.ke booth pameran Pusat Penelitian Bioteknologi-LIPI dalam bazzar Science Week Fair IPH LIPI, Cibinong, 4 September 2017. Tak lupa pada kesempatan ini Bapak Direktur berfoto bersama dengan Plt. Deputi Bidang Jasa Ilmiah LIPI, Dr. Mego Pinandito M.Eng., Kepala Bidang Pengelolaan dan Diseminasi Hasil Penelitian Puslit Bioteknologi-LIPI, Dr. Ir. Syamsidah Rahmawati, serta para peneliti senior Bidang Peternakan Puslit Bioteknologi-LIPI, Prof. Dr. Endang Tri Margawati dan Dr. Ekayanti Mulyawati Kaiin.
  • Penandatangan Naskah Non Disclosure Agreement (NDA) antara Pusat Penelitian Bioteknologi-LIPI dengan PT Phapros Tbk yang dilaksanakan di Cibinong, 4 September 2017. Pada kesempatan ini Pusat Penelitian Bioteknologi LIPI diwakili oleh Kepala Pusat Penelitian Bioteknologi-LIPI, Dr. Bambang Sunarko dan PT Phapros Tbk diwakili oleh Direktur Utama, Barokah Sri Utami, M.M., Apt., serta disaksikan oleh Plt. Kepala LIPI, Prof. Dr. Bambang Subiyanto, Sekretaris Utama LIPI, Dr. Siti Nuramaliati Prijono, Deputi Ilmu Pengetahuan Hayati LIPI, Prof. Dr. Enny Sudarmonowati, dan Kepala Pusat Inovasi LIPI, Dr. Nurul Taufiqu Rochman, M.Eng. Penandatangan NDA ini merupakan salah satu kegiatan dalam rangkaian acara Science Week Fair Kedeputian Ilmu Pengetahuan Hayati LIPI, 4-9 September 2017.
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ROFERON-A: A BIOLOGIC PRODUCT OF HUMAN INTERFERON ALPHA 2A

on .

Andri Wardiana, Ratih Asmana Ningrum (2015).

ROFERON-A: A BIOLOGIC PRODUCT OF HUMAN INTERFERON ALPHA 2A

Human interferRoferon Aon alpha 2a (hIFNα2a) is a cytokine regulating immune system that has been used in hepatitis and cancer treatments. It has wide biological potency covering antiviral, antiproliferative and immunomodulative activities. This mini review discusses Roferon-A as a prominent commercial product of recombinant hIFNα2a which is produced in bacterial system, Escherichia coli, as therapeutic protein for several diseases, such as chronic viral Hepatitis B, Hepatitis C, melanoma, hairy cell leukemia and renal cell carcinoma. The discussion focuses on the development process with regard to its manufacturing, preclinical and clinical studies, as well as therapeutic efficacy. In addition, we also discuss biosimilar development of hIFNα2a and its potential future developments in the context of enhancing pharmacokinetic profiles.

Keywords: interferon alpha 2a, Roferon-A, therapeutic protein and biosimilar.

Sumber: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3176&bid=15625

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Removing a Cystein Group On Interferon Alpha 2b at Position 2 and 99 does Not Diminish Antitumor Activity of the Protein, Even Better

on .

Heni Rachmawati, Adhitya Jessica, Yeyet Cahyati Sumirtaputra, Debbie Sofie Retnoningrum, Amirah Adlia, Ratih Asmana Ningrum (2016).

Removing a CysteinRemoving a Cystein Group On Interferon Alpha 2b at Position 2 and 99 does Not Diminish Antitumor Activity of the Protein Even Better Group On Interferon Alpha 2b at Position 2 and 99 does Not Diminish Antitumor Activity of the Protein, Even Better.

Scientia Pharmaceutica. 84: 113–130. ISSN 0036-8709 (Print) ISSN 2218-0532 (Online) ISSN-L 0036-8709

Interferon alpha 2b is the only standard therapeutic protein for hepatitis virus infections. Further study demonstrated that this protein also posseses antitumor activity in several cancerous organs. One main pathway of this antitumor activity is mediated through antiproliferation as well as proapoptotic effects. Previously, we have successfully developed recombinant human interferon alpha 2b (rhIFNá2b) by using a synthetic gene. In addition, two mutein forms of rhIFNá2b were generated to improve the characteristics of this protein. Two point mutations showed better pharmacokinetic profiles than one point mutation as well as the native form. In the present study, this mutein form was studied for ist antitumor effect in vitro using HepG2 cells. As a comparison, the native form as well as a commercial rIFNá2b were used. Several parameters were investigated including the MTT assay, cell viability test, cell cycle using flow cytometric analysis, and the genes and protein expressions involved in cell growth. The latest was observed to study the mechanism of rhIFNá2b. There was no significant difference in the MTT assay and cell viability after cells were treated with both forms of rhIFNá2b. However, the mutein rhIFNá2b tended to show better proapoptotic activity reflected by flow cytometric data, protein expression of pSTAT1, and DNA expression of caspase 3.

Keywords: Interferon alpha 2b, Antiproliferation, Apoptosis, p21k1, p27, Caspase 3, pSTAT1, Flow cytometri, Mutein

Sumber: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3022&bid=15484

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Glucoamylase Production by Aspergillus awamori KT-11 In Solid-State Fermentation Using Cassava Peel as Substrate

on .

Urip Perwitasari, Nuryati, Ruth Melliawati, and Yopi (2017). Glucoamylase Production by Aspergillus awamori KT-11 In Solid-State Fermentation Using Cassava Peel as Substrate. Annales Bogorienses, Vol. 21 (1): 21-28. ISSN 0517-8452

Glucoamylase Production by Aspergillus awamori KT-11 In Solid-State Fermentation Using Cassava Peel as Substrate

Cassava has long been known as one of the main staple food in Indonesia. Whereas the cassava peel contains starch of approximately 72%, it is still underrated as a carbohydrate source for fermentation. The utilization of cassava peel as a substrate in solid state fermentation potentially replaces rice as a carbon source leading to more cost-effective production. This study aims at producing glucoamylase by means of solid state fermentation using Aspergillus awamori KT-11 and cassava peel as substrate. The study demonstrated that medium composition and drying technique affected the production of glucoamylase. The highest glucoamylase activities were identified when cassava peel and mineral media was used in fermentation, compared to only cassava peel; the combination of cassava peel, mineral, and rice bran; rice media or a mixture of rice, mineral and rice bran. Freeze-dried glucoamylase, furthermore, exhibited higher specific activity in contrast to the oven-dried one, with 452 U/mL and 365 U/mL, respectively. In conclusion, cassava peel plus mineral is a better substrate for glucosamine production by A. awamori KT-11 in solid state fermentation. Besides, powdered glucoamylase had been demonstrated to be capable of hydrolyzing starch-based biomass.

Keywords: glucoamylase, cassava peel, A. awamori KT-11, solid state fermentation

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